解剖学和形态学
麻醉学
听力与言语-语言病理学
行为科学
心脏和心血管系统
细胞和组织工程学
临床神经病学
危重症监护医学
牙科,口腔外科和医学
皮肤病学
急诊医学
内分泌学和新陈代谢
肠胃学和肝脏学
老人病学和老年医学
卫生保健科学和服务
血液学
免疫学
传染病
综合和补充性医学
医学伦理学
医学信息学
医学实验室技术
医学,全科和内科
医学,法律
医学,研究和试验
神经系统科学
护理
营养学和饮食学
产科医学和妇科医学
肿瘤学
眼科学
整形外科学
耳鼻喉科学
病理学
儿科学
周围血管疾病
药理学和药剂学
生理学
基本医疗保健
精神病学
公共、环境和职业卫生
放射学,核医学和医学成像
康复学
生殖生物学
呼吸系统
风湿病学
运动科学
外科学
毒理学
热带医学
泌尿学和肾脏学
病毒学
老年医学
健康政策和服务
心理学,临床
abstract::Two patients with progressive hairy cell leukemia following splenectomy were treated with low-dose daily chlorambucil. Both had an objective hematologic response as determined by a return to normal hematocrit and platelet count. This was also reflected in the mononuclear cell fraction by the normalization of cholester...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1982-07-01 00:00:00
abstract::Although it is recognized that red cells lose membrane during storage, estimation of the osmotic fragility of erythrocytes has not previously proven to be a useful measurement of the storage lesion. Erythrocytes from blood stored in CPD-A2 were found to have a markedly increased osmotic fragility. A major portion of t...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1982-06-01 00:00:00
abstract::Perfluorocarbons have shown promise as clinical blood substitutes. Although early experience in Japan with one such product--Fluosol-DA--has been uncomplicated, we observed an adverse pulmonary reaction in the first American patient to receive it and know of similar reactions in two other Americans so treated. Postula...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1982-06-01 00:00:00
abstract::A high proportion of carriers of classic hemophilia can be identified in the laboratory because, in comparison to normal women, the concentration of antigens related to antihemophilic factor (AHF, factor VIII) that are detected in their plasma by heterologous antiserum (factor VIIIR:Ag) is relatively higher than the t...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1982-06-01 00:00:00
abstract::Incubation of normal human plasma with dextran sulfate for 7 min at 4 degrees C generates kallikrein amidolytic activity. No kallikrein activity is generated in factor XII or prekallikrein-deficient plasma and only small amounts (8%) in high molecular weight (HMW) kininogen-deficient plasma. Addition of specific antis...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1982-06-01 00:00:00
abstract::Platelet-associated C3 (PA-C3) was measured with a quantitative immunofluorescence assay. With this assay, PA-C3 levels were determined for 78 normal volunteers, 30 patients with immune thrombocytopenic purpura (ITP), and 20 patients with nonimmune thrombocytopenias. Platelet-associated IgG (PA-IgG) levels were also m...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1982-05-01 00:00:00
abstract::Time-lapse videotape recordings of polymorphonuclear leukocytes (PMNs) from clot preparations were used to quantify the locomotive behavior of individual PMNs from normal subjects. Tracings derived from the videotapes allow one to quantify multiple parameters of the locomotive behavior of PMNs--direction, distance, ra...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1982-05-01 00:00:00
abstract::We have studied the antileukemic effects of cyclosporin-A (CyA) on the cells of a number of human hematopoietic biopsies and established cell lines in vitro. Cya killed the tumor cells but not the nonmalignant control cells from three of four patients with T-lymphocytic leukemia/lymphoma at concentrations comparable t...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1982-05-01 00:00:00
abstract::Two distinct classes of granulocyte progenitor cells present in normal mouse bone marrow are expressed sequentially in the vivo plasma clot diffusion chamber culture system. By several criteria, progenitor cells giving rise to granulocyte colonies on day 4 of culture (CFU-D4) are different from those giving rise to co...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1982-04-01 00:00:00
abstract::Mice injected chronically with antiplatelet serum develop an increase in the number of megakaryocytic progenitor cells compared to animals given normal rabbit serum. To examine the specificity of this response, progenitor cells giving rise to megakaryocyte, granulocyte-macrophage, erythroid, and mixed-cell colonies we...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1982-04-01 00:00:00
abstract::There is evidence from clinical studies and animal experiments that aspirin has a greater antithrombotic activity in males compared to females. We investigated platelet function in vitro and in vivo in rabbits before and after the administration of a dose of aspirin (5 mg/kg) which inhibited collagen stimulated thromb...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1982-03-01 00:00:00
abstract::One of the most serious complications of successful treatments for Hodgkin's disease is an increased incidence of acute nonlymphocytic leukemia (ANLL) and other malignancies. A retrospective analysis carried out on 1032 consecutive patients with Hodgkin's disease admitted to our Institute between 1965 and 1978 and tre...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1982-03-01 00:00:00
abstract::The presence of lysosomal enzymes in human platelets is well documented; the identity of the "lysosome," however, has been the subject of some disagreement. In order to determine the time of appearance and subcellular localization of two lysosomal enzymes in megakaryocytes (MK) and platelets, we examined normal human ...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1982-03-01 00:00:00
abstract::Radioiodinated L-cell-derived colony-stimulating factor (CSF) was used to characterize the binding reaction to murine bone marrow cells. The major increment in cell-associated radioactivity occurred over 24 hr incubation at 37 degrees C, but virtually no binding was observed at 4 degrees C. The reaction was saturable ...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1982-02-01 00:00:00
abstract::The cellular constituents in lymph nodes and spleens of patients with Hodgkin's disease were studied with a series of monoclonal antibodies directed against human thymocyte, peripheral T-cell, and la antigens. Utilizing both an immunoperoxidase technique on frozen tissue sections and indirect immunofluorescence on cel...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1982-02-01 00:00:00
abstract::Large granular lymphocytes (LGL) are defined as nonadherent mononuclear cells with cytoplasmic azurophilic granules, avid receptors for the Fc portion of IgG, and cytotoxic functions (NK or ADCC activities). In the present study, the granules of LGL isolated from human peripheral blood have been analyzed by enzyme cyt...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1982-02-01 00:00:00
abstract::A cytologic and cytogenetic study of 10 cases of acute myeloblastic leukemia with maturation and t(8;21) translocation is reported. Despite a certain polymorphic appearance, the characteristic cytologic picture, consisting essentially of large myeloblasts with an abundant cytoplasma containing a large Auer rod, allowe...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1982-01-01 00:00:00
abstract::We report a patient with a disease characterized by proliferation of T cells with Fc receptors for IgG (TG). However, unlike lymphoid cells from normal individuals or from patients with other lymphoid malignancies, the patient's lymphocytes spontaneously produced gamma interferon (IFN-gamma) in vitro. The peripheral l...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1982-01-01 00:00:00
abstract::K-562 human leukemia cells grown in the presence of specific goat anti-K-562 gamma globulin, F(ab)'2, Fab', or Fab showed a decrease in DNA and protein syntheses and a loss of cell viability within several hours. Eventually all cells died and lysed within 2-5 days. These events occurred in the absence of added effecto...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1982-01-01 00:00:00
abstract::Washed human platelets were labeled with 125I by the lactoperoxidase-catalyzed method and solubilized in 1% Triton X-100. The soluble proteins were analyzed by crossed-immunoelectrophoresis in 1% agarose, employing a rabbit antibody raised against whole human platelets. Analysis of autoradiograms developed from dried ...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1981-12-01 00:00:00
abstract::von Willebrand's antigen II (vW AgII), a plasma and platelet antigen immunologically and biochemically distinct from factor-VIII-related antigen (VIIIR:Ag), is decreased in von Willebrand's disease. vW AgII is released from platelets during aggregation and clotting. The release of platelet vW AgII was studied in washe...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1981-12-01 00:00:00
abstract::Using either hexazotized pararosaniline or new fuchsin as coupling agents, we investigated the ultrastructural localization of alpha-naphthyl acetate esterase (ANAE) activity in guinea pig bone marrow and peritoneal exudates, and in human peripheral blood cells. DFP-inhibitable ANAE activity was present on the cell su...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1981-12-01 00:00:00
abstract::Two fractions of human prothrombin can be isolated from single donor plasma by the technique of heparin-agarose chromatography in (sodium) citrate buffer, pH 7.5, as previously reported for pooled plasma. The two fractions, designated H-II1 and H-II2, are found in a ratio of approximately 4:1. Both forms comigrate in ...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1981-11-01 00:00:00
abstract::A method is described that allows for the routine long-term (greater than 3 mo) growth of normal, immature human myeloid cells in liquid suspension culture. The techniques employ cell separation, utilization of special growth conditions (RPMI with hydrocortisone and vitamin D), and cord blood as the source of leukocyt...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1981-11-01 00:00:00
abstract::Eighteen patients with agnogenic myeloid metaplasia with myelofibrosis were studied for clinical and laboratory evidence of immunologic dysfunction. Clinical findings included the presence of arthritis, vasculitis, and erythema nodosum. Laboratory abnormalities included the presence of circulating immune complexes, an...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1981-11-01 00:00:00
abstract::We performed a pilot study to test the effectiveness of allogeneic bone marrow transplantation in the treatment of chronic myelogenous leukemia. Five patients in the advanced stages of chronic myelogenous leukemia (four in blast crisis, one in accelerated phase) with abnormal chromosomes underwent matched-sibling allo...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1981-11-01 00:00:00
abstract::Clinical and cytogenetic findings in 10 patients with deletions of the long arm of chromosomes 5 (5q-) are reported. Five cases had refractory anemia, the preleukemic syndrome, or refractory anemia with an excess of blasts; in all but one, the 5q- was the single initial abnormality. Three patients had overt leukemia; ...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1981-11-01 00:00:00
abstract::A 9-yr-old female presented with a Wilm's tumor and a coagulopathy consistent with von Willebrand's disease. Factor VIII procoagulant activity (VIII C), factor VIII related antigen (VIIIR:Ag), and von Willebrand factor activity (VIII:vWf) were decreased. There was no evidence for a circulating inhibitor of the factor ...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1981-10-01 00:00:00
abstract::Three recipients of HLA-identical bone marrow transplants developed chronic graft-versus-host disease (cGVHD) and hypergammaglobulinemia. All three had evidence of abnormal B-lymphocyte function, including a polyclonal increase in immunoglobulins (Ig), antinuclear antibodies, rheumatoid factor, lymphocytotoxins, and i...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1981-10-01 00:00:00
abstract::A specially designed rotational viscometer was used to investigate the effects of the antiplatelet agent PGI2 in combination with theophylline on the response of human platelets subjected to shear stress. Samples of citrated platelet-rich plasma (PRP) were exposed to shear stress in the viscometer for a period of 5 mi...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1981-10-01 00:00:00
abstract::The animal assay of potential new iron-chelating agents is at present dependent on cumbersome and imprecise iron balance studies in hypertransfused rodents. We report the development of a radioisotope assay in intact rats based on the transient labeling by ferritin 59Fe of the main source of chelatable iron within hep...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1981-10-01 00:00:00
abstract::Hemoglobin Great Lakes, beta 68 (E12) Leu replaced by His is a new high oxygen affinity hemoglobin variant discovered in a 29-yr-old female having numerous hospitalizations for thrombophlebitis associated with mild erythrocytosis. The mutant hemoglobin has normal stability and normal electrophoretic mobility, but incr...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1981-10-01 00:00:00
abstract::Sequential immunologic, cytochemical, and cytokinetic studies were done on single lymphoid cells in the peripheral blood of 6 children with infectious mononucleosis (IM) and 1 child with toxoplasmosis 1-2 wk after onset of symptomatology. The absolute number of AET-SRBC rosetting (E+) cells was increased in all patien...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1981-09-01 00:00:00
abstract::Whether platelet volume increases during the morphological changes preceding aggregation has been investigated. Previous research is controversial; resistive-counting techniques reveal an increase, centrifugal methods do not. Platelets were sized with a computerized, resistive-particle counter before and after incubat...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1981-09-01 00:00:00
abstract::The addition of a T-cell growth-promoting medium (PHA-TCM) to culture conditions that support growth of multi-lineage hemopoietic colonies enhances the proliferation of cells with lymphoid morphology within these colonies. These cells were identified as T lymphocytes by their ability to form rosettes with SRBC and the...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1981-08-01 00:00:00
abstract::Autologous membrane-bound IgG was isolated from a subpopulation of human red blood cells (RBC) with specific density greater than 1.110, by affinity chromatography of purified RBC membrane glycoprotein preparations using immobilized wheat germ agglutinin and immobilized anti-human immunoglobulin (Ig) as immunoabsorben...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1981-08-01 00:00:00
abstract::Hemoglobin and ferritin iron content have been followed during differentiation in tissue cultures of murine erythroleukemia cells (MELC) using the techniques of Mössbauer spectroscopy and electron microscopy. In undifferentiated cells grown without DMSO, only iron stored in ferritin was detected. The amount of iron in...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1981-08-01 00:00:00
abstract::We have studied the effect of prostaglandin I2 on platelet turnover, attachment to the subendothelium, and secretion following balloon deendothelialization of the rabbit aorta. Survival of 51Cr-labeled platelets in the balloon-injured animals remained normal. Thirty minutes after injury, there were 4.52 X 10(6) platel...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1981-08-01 00:00:00
abstract::Biochemical and morphological evidence is presented to support the characterization of a rat bone-marrow-derived cell line (RPM) as an analog of the promegakaryoblast. The conditions for in vitro growth and maturation of the RPM line are described. Rapid proliferation of the RPM line is readily achieved when cultures ...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1981-07-01 00:00:00
abstract::The relationship between neutrophil function and the neutrophil antigen, Kx, as well as the linkage of the gene, Xk, with Xg was examined in a kindred with X-linked chronic granulomatous disease. Four of the eight male siblings had chronic granulomatous disease by clinical history and tests of neutrophil function, and...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1981-07-01 00:00:00